ABSTRACT
<p><b>OBJECTIVE</b>To determine the reversal effect of aloe emodin liposomes (AE-L) on cisplatin resistance human lung adenocarcinoma cell line A549/DDP.</p><p><b>METHOD</b>The un-cytotoxic and cytotoxic concentration of AE-L, and un-cytotoxic concentration of E-L were determined by MTT. The sensitivity of cisplatin were determined by MTT assay in above 3 groups. The intracellular concentration of cisplatin was detected by inductively coupled plasma mass spectrometry (ICP-MS).</p><p><b>RESULT</b>The maximum non-toxic concentration group of AE-L (2.0 mg x L(-1)) increased the sensitivity of cisplatin in A549/DDP, decreased IC50 of cisplatin in A549/DDP from 16.81 mg x L(-1) to 5.86 mg x L(-1), and the hyp-cytotoxic concentration (7.0 mg x L(-1) ) group's IC50 decreased to 4.34 mg x L(-1); AE-L groups significantly increased intracellular concentration of cisplatin in A549/DDP cells.</p><p><b>CONCLUSION</b>The results showed that aloe emodin can reverse multidrug resistance (MDR) of A549/DDP cells and the mechanism might be associated with the increase of intracellular concentration of cisplatin.</p>
Subject(s)
Animals , Humans , Adenocarcinoma , Pathology , Anthraquinones , Chemistry , Pharmacology , Cell Line, Tumor , Cisplatin , Pharmacology , Drug Resistance, Multiple , Drug Resistance, Neoplasm , Inhibitory Concentration 50 , Lethal Dose 50 , Liposomes , Lung Neoplasms , PathologyABSTRACT
<p><b>OBJECTIVE</b>To establish a simple and rapid HPLC-MS method for determining the contents of olmesartan in human plasma.</p><p><b>METHODS</b>Plasma were precipitated with trifluoroacetic acid, then analyzed on an HyPurity C(18) column (150 mm 2.1 mm, 5 microm). Samples at 40 degrees celsius;. The mobile phase consisted of water-methanol- acetonitrile(14:60:26) with a flow rate of 0.22 ml/min.</p><p><b>RESULTS</b>The lower limit of qualification was 25 microg/L. The calibration curve was linear over the range of 25-3200 microg/L (r=0.9998), with the intra-day and inter-day RSD less than 15%.</p><p><b>CONCLUSION</b>The method is sensitive, rapid and suitable for the study of pharmacokinetics and bioavailability of olmesartan.</p>